diff --git a/etc/evergreen_yml_components/definitions.yml b/etc/evergreen_yml_components/definitions.yml
index 17aeed36853..3f91f4a8b48 100644
--- a/etc/evergreen_yml_components/definitions.yml
+++ b/etc/evergreen_yml_components/definitions.yml
@@ -270,6 +270,8 @@ functions:
     display_name: "adjust venv"
     params:
       binary: bash
+      env:
+        use_python310: ${use_python310|}
       args:
         - "src/evergreen/functions/venv_adjust.sh"
 
@@ -788,6 +790,8 @@ functions:
     display_name: "set up venv"
     params:
       binary: bash
+      env:
+        use_python310: ${use_python310|}
       args:
         - "src/evergreen/functions/venv_setup.sh"
 
diff --git a/etc/evergreen_yml_components/variants/amazon/test_release.yml b/etc/evergreen_yml_components/variants/amazon/test_release.yml
index b5e4280b428..de83ec146ae 100644
--- a/etc/evergreen_yml_components/variants/amazon/test_release.yml
+++ b/etc/evergreen_yml_components/variants/amazon/test_release.yml
@@ -11,6 +11,8 @@ buildvariants:
     run_on:
       - amazon2-latest-small
     expansions:
+      # TODO SERVER-119677: Remove use_python310 once Amazon2 sqlite is upgraded
+      use_python310: true
       test_flags: >-
         --excludeWithAnyTags=SERVER-34286,incompatible_with_amazon_linux,requires_external_data_source
         --modules=none
@@ -63,6 +65,8 @@ buildvariants:
       - amazon2-latest-small
     tags: ["emergency_release"]
     expansions:
+      # TODO SERVER-119677: Remove use_python310 once Amazon2 sqlite is upgraded
+      use_python310: true
       additional_package_targets: >-
         archive-mongocryptd-stripped
         archive-mongocryptd-debug
@@ -116,6 +120,8 @@ buildvariants:
     run_on:
       - amazon2-arm64-latest-m8g-xlarge
     expansions:
+      # TODO SERVER-119677: Remove use_python310 once Amazon2 sqlite is upgraded
+      use_python310: true
       test_flags: >-
         --excludeWithAnyTags=SERVER-34286,incompatible_with_amazon_linux,requires_external_data_source
         --modules=none
@@ -168,6 +174,8 @@ buildvariants:
       - amazon2-arm64-latest-m8g-xlarge
     tags: ["emergency_release"]
     expansions:
+      # TODO SERVER-119677: Remove use_python310 once Amazon2 sqlite is upgraded
+      use_python310: true
       additional_package_targets: >-
         archive-mongocryptd-stripped
         archive-mongocryptd-debug
diff --git a/evergreen/prelude_python.sh b/evergreen/prelude_python.sh
index 200fd7f0269..0d85cbc7630 100644
--- a/evergreen/prelude_python.sh
+++ b/evergreen/prelude_python.sh
@@ -5,8 +5,18 @@ elif [ "$(uname)" = "Darwin" ]; then
     python='/Library/Frameworks/Python.Framework/Versions/3.13/bin/python3'
     echo "Executing on mac, setting python to ${python}"
 else
+    # Check if Python 3.10 is requested via Evergreen parameter
+    # TODO SERVER-119677: Remove use_python310 parameter once Amazon2 sqlite is upgraded
+    if [ "${use_python310}" = "true" ]; then
+        if [ -f /opt/mongodbtoolchain/v4/bin/python3 ]; then
+            python="/opt/mongodbtoolchain/v4/bin/python3"
+            echo "Using Python 3.10 from v4 toolchain (use_python310=true): ${python}"
+        else
+            echo "ERROR: use_python310 is set but Python 3 is not available at /opt/mongodbtoolchain/v4/bin/python3"
+            return 1
+        fi
     # Check if v5 toolchain exists - it requires Python 3.13
-    if [ -d /opt/mongodbtoolchain/v5 ]; then
+    elif [ -d /opt/mongodbtoolchain/v5 ]; then
         if [ -f /opt/mongodbtoolchain/v5/bin/python3.13 ]; then
             python="/opt/mongodbtoolchain/v5/bin/python3.13"
             echo "Found python 3.13 in v5 toolchain, setting python to ${python}"